S100β Polyclonal Antibody-BS91198

S100β Polyclonal Antibody Sizes: 50µl, 100µl Catalogue Numbers: BS91198-50, BS91198-100 Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Swiss-Prot: P04271 Host: Rabbit Reactivity: Human, Mouse, Rat, Goat, zebrafish Applications: WB, IF, IP, IHC All Applications: WB:1:500-1:2000 IF:1:50-1:200 IHC:1:50-1:200 IP:1:50-1:200 Background: The family of EF-hand type Ca2⁺-binding proteins includes calbindin (previously designated vitamin D-dependent Ca2⁺-binding protein), S-100 α and β, calgranulins A (also designated MRP8), B (also designated MRP14) and C (S-100 like proteins), and the parvalbumin family members, including parvalbumin α and parvalbuminβ(also designated oncomodulin). The S-100 protein is involved in the regulation of cellular processes such as cell cycle progression and differentiation. Research also indicates that the S-100 protein may function in the activation of Ca2⁺ induced Ca2⁺ release, inhibition of microtubule assembly and inhibition of protein kinase C mediated phosphorylation. Two S-100 subunits, sharing 60% sequence identity, have been described as S-100 α chain and S-100 β chain. Three S-100 dimeric forms have been characterized, differing in their subunit composition of either two α chains, two β chains or one α and one β chain. S-100 localizes to the cytoplasm and nuclei of astrocytes, Schwann's cells, ependymomas and astrogliomas. S-100 is also detected in almost all benign naevi, malignant melanocytic tumours and in Langerhans cells in the skin. Calbindin, S-100 proteins and parvalbumin proteins are each expressed in neural tissues. In addition, S-100 α and β are present in a variety of other tissues, and calbindin is present in intestine and kidney. Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. Specificity: S100β polyclonal antibody detects endogenous levels of S100β protein. Bioworld Molecular Weight: ~ 11 kDa Note: For research use only, not for use in diagnostic procedure. Extra Notes: Western blot analysis of extracts of various cell lines, using S100β pAb at 1:1000 dilution., Immunohistochemistry of paraffin-embedded Mouse brain using S100β pAb at dilution of 1:100 (40x lens)., Immunofluorescence analysis of U-251 MG cells using S100β pAb at dilution of 1:100 (40x lens)., Immunoprecipitation analysis of 600ug extracts of Mouse brain cells using 3ug S100β antibody. Western blot was performed from the immunoprecipitate using S100β antibody at a dilition of 1:500. Alternative Name: NEF; S100; S100-B; S100beta; S100B; S100 beta Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-92 of human S100B (NP_006263.1). Conjugate: Unconjugated Modification: Unmodified