S100-A2 Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB66956-50, MB66956-100
Product: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Swiss-Prot: P29034
Host: Mouse
Reactivity: Human
Applications: WB, IHC, IF/ICC
All Applications: WB (1/500 - 1/2000), IHC (1/50 - 1/200), IF/ICC (1/10 - 1/50)
Background: May function as calcium sensor and modulator, contributing to cellular calcium signaling. May function by interacting with other proteins, such as TPR-containing proteins, and indirectly play a role in many physiological processes. May also play a role in suppressing tumor cell growth.
Purification and Purity: This antibody is purified through a protein G column.
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Recognizes endogenous levels of S100-A2 protein.
Bioworld Molecular Weight: ~ 12 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of S100-A2 expression in A431 (A), HACAT (B), SW480 (C) whole cell lysates., Immunohistochemical analysis of S100-A2 staining in human skin formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX., Immunofluorescent analysis of S100-A2 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain the cytoplasm (red). DAPI was used to stain the cell nuclei (blue).
Alternative Name: S100L; Protein S100-A2; CAN19; Protein S-100L; S100 calcium-binding protein A2
Immunogen: Recombinant fusion protein of human S100-A2. The exact sequence is proprietary.
Conjugate: Unconjugated
Modification: Unmodified