Product Description

S6K1 Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS67442-50, BS67442-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: p70 S6 kinase is a mitogen activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression . p70 S6 kinase phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5' oligopyrimidine tract mRNAs. A second isoform, p85 S6 kinase, is derived from the same gene and is identical to p70 S6 kinase except for 23 extra residues at the amino terminus, which encode a nuclear localizing signal . Both isoforms lie on a mitogen activated signaling pathway downstream of phosphoinositide-3 kinase (PI-3K) and the target of rapamycin, FRAP/mTOR, a pathway distinct from the Ras/MAP kinase cascade . The activity of p70 S6 kinase is controlled by mμltiple phosphorylation events located within the catalytic, linker and pseudosubstrate domains . Phosphorylation of Thr229 in the catalytic domain and Thr389 in the linker domain are most critical for kinase function . Phosphorylation of Thr389, however, most closely correlates with p70 kinase activity in vivo . Prior phosphorylation of Thr389 is required for the action of phosphoinositide 3-dependent protein kinase 1 (PDK1) on Thr229 . Phosphorylation of this site is stimμlated by growth factors such as insμlin, EGF and FGF, as well as by serum and some G-protein-coupled receptor ligands, and is blocked by wortmannin, LY294002 (PI-3K inhibitor) and rapamycin (FRAP/mTOR inhibitor) . Ser411, Thr421 and Ser424 lie within a Ser-Pro-rich region located in the pseudosubstrate region . Phosphorylation at these sites is thought to activate p70 S6 kinase via relief of pseudosubstrate suppression . Another LY294002 and rapamycin sensitive phosphorylation site, Ser371, is an in vitro substrate for mTOR and correlates well with the activity of a partially rapamycin resistant mutant p70 S6 kinase.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat, Bovine

Host: Rabbit

Applications: WB, IHC

Alternate Names: STK14A, Ribosomal protein S6 kinase beta-1, S6K-beta-1, S6K1, 70 kDa ribosomal protein S6 kinase 1, P70S6K1, p70-S6K 1, Ribosomal protein S6 kinase I, Serine/threonine-protein kinase 14A, p70 ribosomal S6 kinase alpha, p70 S6 kinase alpha, p70 S6K-alpha, p70 S6KA

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human S6K1. The exact sequence is proprietary.

Dilution: WB (1/500 - 1/1000), IHC (1/50 - 1/200)

Purification: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of S6K1 protein.

Molecular Weight: ~ 70 kDa

Pathway: AMPK Signaling Pathway, ErbB HER Signaling, Inhibition of Apoptosis, Mitochondrial Control of Apoptosis, PI3K AKT signalingm, Protein Kinase C Signaling, TGFβ Smad signaling, Translational Contral Regμlation of elF2, MTOR signaling.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P23443

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Research Use Only