Product Description

SDHB Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES4180-50, ES4180-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in this gene result in paragangliomas and pheochromocytoma, and support a link between mitochondrial dysfunction and tumorigenesis. [provided by RefSeq, Jul 2008],

Reactivity: Human, Mouse, Rat, Fish

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: SDHB, SDH, SDH1, Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial, Iron-sulfur subunit of complex II, Ip

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from the Internal region of human SDHB. AA range:131-180

Isotype: IgG

Target: SDHB

Specificity: SDHB Polyclonal Antibody detects endogenous levels of SDHB protein.

Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Brain, Fibroblast, Liver,

Observed Band (KD): 31kD

Fields: Citrate cycle (TCA cycle), Oxidative phosphorylation, Metabolic pathways, Carbon metabolism, Thermogenesis, Non-alcoholic fatty liver disease, Alzheimer disease, Parkinson disease, Amyotrophic lateral sclerosis, Huntington disease, Prion disease, Pathways of neurodegeneration - multiple diseases, Chemical carcinogenesis - reactive oxygen species, Diabetic cardiomyopathy

Function: Catalytic Activity: Succinate + ubiquinone = fumarate + ubiquinol
Cofactor: Binds 1 2Fe-2S cluster
Cofactor: Binds 1 3Fe-4S cluster
Cofactor: Binds 1 4Fe-4S cluster
Disease: Defects in SDHB are a cause of Cowden-like syndrome [MIM: 612359]. Cowden-like syndrome is a cancer predisposition syndrome associated with elevated risk for tumors of the breast, thyroid, kidney and uterus
Disease: Defects in SDHB are a cause of paraganglioma and gastric stromal sarcoma [MIM: 606864], also called Carney-Stratakis syndrome. Gastrointestinal stromal tumors may be sporadic or inherited in an autosomal dominant manner, alone or as a component of a syndrome associated with other tumors, such as in the context of neurofibromatosis type 1 (NF1). Patients have both gastrointestinal stromal tumors and paragangliomas. Susceptibility to the tumors was inherited in an apparently autosomal dominant manner, with inc

Subcellular Location: Mitochondrion inner membrane, Peripheral membrane protein, Matrix side

Storage and Stability: -20°C/1 year

Gene Name: SDHB

Protein Name: Succinate dehydrogenase [ubiquinone] iron-sulfur subunit mitochondrial

Mouse Gene ID: 67680

Mouse SwissProt Number: Q9CQA3

Rat Gene ID: 298596

Rat SwissProt Number: P21913

Research Use Only