Product Description

SETDB1 Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS7938-50, BS7938-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: ERG-associated protein with SET domain (ESET), also designated Histone H3-K9 methyltransferase 4 or SET domain bifurcated 1, is a nuclear protein belonging to the histone-lysine methyltransferase family and to the Suvar3-9 subfamily. It is a highly conserved protein of 150 amino acids that has been implicated in chromatin structure modμlation. ESET is excluded from cell nucleoli and areas of condensed chromatin and can associate with the nonpericentromeric regions of chromatin. The gene encoding for this protein, SETDB1, maps to chromosome 1q21. ESET is a histone methyltransferase, methylating Lys-9 of histone H3 and mutations within the SETDB1 gene abolishes its methyltransferase activity. This methylation acts as a tag for epigenetic transcriptional repression by rounding up HP1 proteins to methylated histones. ESET is widely expressed with highest levels found in testis.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF

Alternate Names: ERG-associated protein with SET domain, ESET, H3 K9 HMTase 4, H3 lysine 9 specific 4, H3-K9-HMTase 4, H3-K9-HMTase4, Histone H3 K9 methyltransferase 4, Histone H3-K9 methyltransferase 4, Histone lysine N methyltransferase, Histone-lysine N-methyltransferase SETDB1, KG1T, KMT1E, Lysine N-methyltransferase 1E, MGC90670, mKIAA0067, SET domain bifurcated 1, Set domain protein, bifurcated, 1, SETB, SETB1_HUMAN, SETDB1, Similar to DNA topoisomerase II,

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant fμll length Human SETDB1.

Dilution: WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: SETDB1 polyclonal antibody detects endogenous levels of SETDB1 protein.

Molecular Weight: ~ 210 kDa

Pathway: DNA Methylation, Epigenetic Writers and Erasers of Histone H3,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q15047

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only