Product Description

Siglec-15 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS80219-50, BS80219-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q6ZMC9

Host: Rabbit

Reactivity: Human, Rat

Applications: WB, IHC, IF/ICC

All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:200 | IF/ICC,1:50 - 1:200

Background: SIGLEC15 (Sialic Acid Binding Ig Like Lectin 15) is a Protein Coding gene. Diseases associated with SIGLEC15 include Osteoporosis, Juvenile and Osteoporosis. Among its related pathways are Innate Immune System and RET signaling. An important paralog of this gene is SIGLEC1.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 36KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using SIGLEC15 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 180s., Immunohistochemistry of paraffin-embedded human spleen using Siglec-15 Rabbit pAb at dilution of 1:100. Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded human esophageal cancer using Siglec-15 Rabbit pAb at dilution of 1:100. Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol., Immunofluorescence analysis of human spleen cells using Siglec-15 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: CD33L3, HsT1361, SIGLEC-15

Immunogen: Recombinant fusion protein of human Siglec-15(NP_998767.1).

Conjugate: Unconjugated

Modification: Unmodified