Simian OC/BGP (Osteocalcin) ELISA Kit - ELK9627

Simian OC/BGP (Osteocalcin) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK9627-48, ELK9627-96, ELK9627-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: BGLAP; BGP; OC; OCN; bone gamma-carboxyglutamate protein; Osteocalcin

Assay Type: Sandwich

Sensitivity: 0.46 ng/mL

Standard: 50 ng/mL

Detection Range: 0.78-50 ng/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Length: 3.5h

Research Areas: Stem Cells; Signal Transduction;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian OC/BGP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian OC/BGP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian OC/BGP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian OC/BGP in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P02819

Research Use Only