Product Description

Simian sC5b-9 (Soluble Terminal Complement Complex C5b-9) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK11958-48, ELK11958-96, ELK11958-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Simian

Alternate Names: MAC, Membrane Attack Complex, C5b-9, Terminal Complement Complex C5b-9, C5b9

Sensitivity: 13.2 ng/mL

Assay Type: Sandwich

Standard: 2000 ng/mL

Detection Range: 31.25-2000 ng/mL

Sample Type: Serum, Plasma, Other Biological Fluids

Assay Length: 3.5h

Research Area: Signal Transduction, Metabolic Pathway, Apoptosis, Infection Immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian sC5b-9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian sC5b-9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian sC5b-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian sC5b-9 in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only