Product Description

SIRT1 Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66593-50, MB66593-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The Silent Information Regμlator (SIR2) family of genes is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases, also known as class III histone deacetylases. The first discovered and best characterized of these genes is Saccharomyces cerevisiae SIR2, which is involved in silencing of mating type loci, telomere maintenance, DNA damage response, and cell aging. SirT1, the mammalian ortholog of Sir2, is a nuclear protein implicated in the regμlation of many cellμlar processes, including apoptosis, cellμlar senescence, endocrine signaling, glucose homeostasis, aging, and longevity. Targets of SirT1 include acetylated p53, p300, Ku70, forkhead (FoxO) transcription factors, PPARγ, and the PPARγ coactivator-1α (PGC-1α) protein. Deacetylation of p53 and FoxO transcription factors represses apoptosis and increases cell survival. Deacetylation of PPARγ and PGC-1α regμlates the gluconeogenic/glycolytic pathways in the liver and fat mobilization in white adipocytes in response to fasting. SirT1 deacetylase activity is inhibited by nicotinamide and activated by resveratrol. In addition, SirT1 activity may be regμlated by phosphorylation, as it is phosphorylated at Ser27 and Ser47 in vivo, however, the function of these phosphorylation sites has not yet been determined.

Category: Primary Antibody

Reactivity: Human

Host: Mouse

Applications: WB, IP

Alternate Names: SIR2L1, NAD-dependent protein deacetylase sirtuin-1, hSIRT1, Regμlatory protein SIR2 homolog 1, SIR2-like protein 1, hSIR2

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Purified recombinant human SIRT1 protein fragments expressed in E.coli.

Dilution: WB (1/500 - 1/1000), IP (1/10 - 1/50)

Purification: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of SIRT1 protein.

Molecular Weight: ~ 120 kDa

Pathway: AMPK Signaling Pathway

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q96EB6

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Research Use Only