Product Description

SIRT1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS80148-50, BS80148-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q96EB6

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IF/ICC, IP, , ChIP,

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200 | IP, 50 - 1:200 | ChIP,1:20 - 1:100

Background: This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 120kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using SIRT1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s., Immunofluorescence analysis of HeLa cells using [KO Validated] SIRT1 Rabbit pAb at dilution of 1:250. Blue: DAPI for nuclear staining., Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] SIRT1 Rabbit pAb at dilution of 1:250. Blue: DAPI for nuclear staining.

Alternative Name: SIR2; SIR2L1; SIR2alpha; SIRT1; sirtuin 1

Immunogen: Recombinant fusion protein of human SIRT1(NP_036370.2).

Conjugate: Unconjugated

Modification: Unmodified