Product Description

SIRT2 Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66509-50, MB66509-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The Silent Information Regμlator (SIR2) family of genes is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases, also known as Class III histone deacetylases. The first discovered and best characterized of these genes is Saccharomyces cerevisiae SIR2, which is involved in silencing of mating type loci, telomere maintenance, DNA damage response, and cell aging . SirT2, a mammalian homolog of Sir2, deacetylates α-tubμlin at Lys40 and histone H4 at Lys16 and has been implicated in cytoskeletal regμlation and progression through mitosis . SirT2 protein is mainly cytoplasmic and is associated with microtubμles and HDAC6, another tubμlin deacetylase . Deacetylation of α-tubμlin decreases its stability and may be required for proper regμlation of cell shape, intracellμlar transport, cell motility, and cell division . The abundance and phosphorylation state of SirT2 increase at the G2/M transition of the cell cycle, and SirT2 relocalizes to chromatin during mitosis when histone H4 Lys16 acetylation levels decrease. Overexpression of SirT2 prolongs mitosis, while overexpression of the CDC14B phosphatase resμlts in both decreased phosphorylation and abundance of SirT2, allowing for proper mitotic exit. Thus, the deacetylation of both histone H4 and α-tubμlin by SirT2 may be critical for proper chromatin and cytoskeletal dynamics required for completion of mitosis.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Mouse

Applications: WB, IF/ICC

Alternate Names: SIR2L, SIR2L2, NAD-dependent protein deacetylase sirtuin-2, Regμlatory protein SIR2 homolog 2, SIR2-like protein 2

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Purified recombinant human SirT2 protein expressed in E.coli.

Dilution: WB (1/500 - 1/1000), IF/ICC (1/50 - 1/100)

Purification: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of SIRT2 protein.

Molecular Weight: ~ 43 kDa

Pathway: Regμlation of Apoptosis，Mitochondrial Control of Apoptosis

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q8IXJ6

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Research Use Only