Product Description

SKAP2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS8215-50, BS8215-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O75563

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

All Applications: WB: 1:500~1:2000
IHC/IF: 1:50~1:200

Background: Fyb (Fyn binding protein) and the anchoring proteins SKAP55 and SKAP55-R (SKAP55-related protein) associate with the tyrosine kinase p59fyn. SKAP55 and SKAP55-R bind to Fyb through their SH3 domains and function as substrates for p59Fyn in resting T cells. SKAP55 contains an N-terminal pleckstrin homology domain and a C-terminal SH3 domain binding motif of adjacent arginine and lysine residues followed by tandem tyrosines (i.e. RKxxYxxY). SKAP55-R, similar in overall structure to SKAP55, contains a coiled-coil N-terminal domain. SKAP55 associates with SLAP-130, another component of the Fyn complex, which plays a role in the regulation of signaling events initiated by lymphocyte antigen receptors leading up to T cell activation. The human SKAP55 gene maps to chromosome 17q21.32 and encodes a 359 amino acid protein.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: SKAP2 polyclonal antibody detects endogenous levels of SKAP2 protein.

Bioworld Molecular Weight: ~ 50 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemistry of paraffin-embedded mouse stomach using SKAP2 antibody at dilution of 1:100 (x40 lens)., Immunohistochemistry of paraffin-embedded rat lung using SKAP2 antibody at dilution of 1:100 (x40 lens)., Immunohistochemistry of paraffin-embedded mouse lung using SKAP2 antibody at dilution of 1:100 (x40 lens)., Western blot analysis of extracts of mouse lung, using SKAP2 antibody.

Alternative Name: Src kinase-associated phosphoprotein 2; Pyk2/RAFTK-associated protein; Retinoic acid-induced protein 70; SKAP55 homolog; SKAP-55HOM; SKAP-HOM; Src family-associated phosphoprotein 2; Src kinase-associated phosphoprotein 55-related protein; Src-associated adapter protein with PH and SH3 domains; SKAP2; PRAP; RA70; SAPS; SCAP2; SKAP55R

Immunogen: Recombinant full length Human SKAP2.

Conjugate: Unconjugated

Modification: Unmodified