Product Description

SMAD3 Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB65975-50, MB65975-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Receptor-regμlated SMAD (R-SMAD) that is an intracellμlar signal transducer and transcriptional modμlator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regμlated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regμlate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modμlating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regμlator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regμlates PDPK1 kinase activity by stimμlating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regμlator.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Mouse

Applications: WB, IHC

Alternate Names: MADH3, Mothers against decapentaplegic homolog 3, MAD homolog 3, Mad3, Mothers against DPP homolog 3, hMAD-3, JV15-2, SMAD family member 3, SMAD 3, Smad3, hSMAD3

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant protein corresponding to human SMAD3.

Dilution: WB (1/1000 - 1/2000), IHC (1/100 - 1/200)

Purification: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Recognizes endogenous levels of SMAD3 protein.

Molecular Weight: ~ 52 kDa

Pathway: Tumor Angiogenesis, Cell Cycle Control G1 S Checkpoint, TGFβ Smad signaling,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P84022

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Research Use Only