Product Description

Smad3 (phospho-S204) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS4874-50, BS4874-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Smad proteins, the mammalian homologs of the Drosophila mothers against decapentaplegic (Mad), have been implicated as downstream effectors of GFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1) and Smad5 are effectors of BMP-2 and BMP-4 function, while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and activin-mediated growth modμlation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regμlate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad proteins.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, ICC, IP

Alternate Names: Mothers against decapentaplegic homolog 3, MAD homolog 3, Mad3, Mothers against DPP homolog 3, hMAD-3, JV15-2, SMAD family member 3, SMAD 3, Smad3, hSMAD3, SMAD3, MADH3

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: Synthetic phosphopeptide derived from human Smad3 around the phosphorylation site of Serine 204.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200
ICC: 1:50~1:200
IP: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-Smad3 (S204) polyclonal antibody detects endogenous levels of Smad3 protein only when phosphorylated at Ser204.

Molecular Weight: ~ 48, 55 kDa

Pathway: Tumor Angiogenesis, Cell Cycle Control G1 S Checkpoint, Contribution of Soluble Factors to EMT, hippo Signaling, TGFβ Smad signaling ,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P84022

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only