Product Description

SMAD3 (phospho-S423) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS64037-50, BS64037-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad), have been implicated as downstream effectors of TGFß/BMP signaling. Smad1 (also designated Madr1 or JV4-1) and Smad5 are effectors of BMP2 and BMP4 function, while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFß and activin-mediated growth modμlation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regμlate the response to activin/TGFß signaling by interfering with TGF∫-mediated phosphorylation of other Smad family members.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB

Alternate Names: Mothers against decapentaplegic homolog 3, MAD homolog 3, Mad3, Mothers against DPP homolog 3, hMAD-3, JV15-2, SMAD family member 3, SMAD 3, Smad3, hSMAD3, SMAD3, MADH3

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: A synthetic peptide corresponding to residues in Human SMAD3 around the phosphorylation site of Ser423.

Dilution: WB: 1:500~1:1000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: SMAD3(p-s423) polyclonal antibody detects endogenous levels of SMAD3 protein only when phosphorylated at ser423.

Molecular Weight: ~ 55 kDa

Pathway: Tumor Angiogenesis, Cell Cycle Control G1 S Checkpoint, Contribution of Soluble Factors to EMT, hippo Signaling, TGFβ Smad signaling ,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P84022

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only