Product Description

SMAD5 Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66501-50, MB66501-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Bone morphogenetic proteins (BMPs) constitute a large family of signaling molecμles that regμlate a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation, and apoptosis . BMP receptors are members of the TGF-β family of Ser/Thr kinase receptors. Ligand binding induces mμltimerization, autophosphorylation, and activation of these receptors . They subsequently phosphorylate Smad1 at Ser463 and Ser465 in the carboxy-terminal motif SSXS, as well as Smad5 and Smad9 (Smad8) at their corresponding sites. These phosphorylated Smads dimerize with the coactivating Smad4 and translocate to the nucleus, where they stimμlate transcription of target genes . MAP kinases and CDKs 8 and 9 phosphorylate residues in the linker region of Smad1, including Ser206. The phosphorylation of Ser206 recruits Smurf1 to the linker region and leads to the degradation of Smad1. Phosphorylation of this site also promotes Smad1 transcriptional action by recruiting YAP to the linker region .

Category: Primary Antibody

Reactivity: Human

Host: Mouse

Applications: WB, IF/ICC, FC

Alternate Names: MADH5, Mothers against decapentaplegic homolog 5, MAD homolog 5, Mothers against DPP homolog 5, JV5-1, SMAD family member 5, SMAD 5, Smad5, hSmad5

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Purified recombinant human SMAD5 (C-terminus) protein fragments expressed in E.coli.

Dilution: WB (1/500 - 1/1000), IF/ICC (1/50 - 1/100), FC (1/50 - 1/100)

Purification: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of SMAD5 protein.

Molecular Weight: ~ 60 kDa

Pathway: ESC Pluripotency and Differentiation Signaling Pathway, TGFβ Smad signaling

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q99717

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Research Use Only