Product Description

SMC1A Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB0047-50, MB0047-100

Product: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Swiss-Prot: Q14683

Host: Mouse

Reactivity: Human

Applications: WB, IHC

All Applications: WB: 1:500
IHC: 1:500

Background: Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interac tion with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.

Purification and Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of SMC1A and does not cross-react with related proteins

Bioworld Molecular Weight: ~ 143 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of SMC1A monoclonal antibody at 1:500 dilution, IHC of paraffin-embedded Human liver tissue using anti-SMC1A diluted 1:500., IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-SMC1A diluted 1:500., IHC of paraffin-embedded Human liver tissue using anti-SMC1A diluted 1:500.

Alternative Name: Structural maintenance of chromosomes protein 1A; SMC protein 1A; SMC-1-alpha; SMC-1A; Sb1.8; SMC1A; DXS423E; KIAA0178; SB1.8; SMC1; SMC1L1

Immunogen: Human recombinant protein fragment corresponding to amino acids 889-1016 of human SMC1A (NP_006297) produced in E.coli.

Conjugate: Unconjugated

Modification: Unmodified