Product Description

SMYD4 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS78344-50, BS78344-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q8IYR2

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IF/ICC, IP,

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200 | IP,1:20 - 1:50

Background: Plays a critical role in cardiac development. Acts as a key epigenetic regulator of gene expression during cardiac development via its dual activities as a methyltransferase and negative regulator of HDAC1 (By similarity.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 90kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using SMYD4 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s., Immunofluorescence analysis of MCF-7 cells using SMYD4 antibody. Blue: DAPI for nuclear staining., Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug SMYD4 antibody. Western blot was performed from the immunoprecipitate using SMYD4 antibody at a dilution of 1:500. Detection: ECL Enhanced Kit.
Exposure time: 20s.

Alternative Name: SMYD4; ZMYND21

Immunogen: Recombinant fusion protein of human SMYD4(NP_443160.2).

Conjugate: Unconjugated

Modification: Unmodified