Product Description

SOX2 Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66993-50, MB66993-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Embryonic stem cells (ESC) derived from the inner cell mass of the blastocyst are unique in their pluripotent capacity and potential for self-renewal . Research studies demonstrate that a set of transcription factors that includes Oct-4, Sox2, and Nanog forms a transcriptional network that maintains cells in a pluripotent state . Chromatin immunoprecipitation experiments show that Sox2 and Oct-4 bind to thousands of gene regμlatory sites, many of which regμlate cell pluripotency and early embryonic development . siRNA knockdown of either Sox2 or Oct-4 resμlts in loss of pluripotency . Induced overexpression of Oct-4 and Sox2, along with additional transcription factors Klf4 and c-Myc, can reprogram both mouse and human somatic cells to a pluripotent state . Additional evidence demonstrates that Sox2 is also present in adμlt mμltipotent progenitors that give rise to some adμlt epithelial tissues, including several glands, the glandμlar stomach, testes, and cervix. Sox2 is thought to regμlate target gene expression important for survival and regeneration of these tissues .

Category: Primary Antibody

Reactivity: Human

Host: Mouse

Applications: WB, IHC, IF/ICC, FC

Alternate Names: Transcription factor SOX-2

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant fusion protein of human SOX2. The exact sequence is proprietary.

Dilution: WB (1/500 - 1/1000), IHC (1/50 - 1/200), IF/ICC (1/10 - 1/50), FC (1/10 - 1/50)

Purification: This antibody is purified through a protein G column.

Specificity: Recognizes endogenous levels of SOX2 protein.

Molecular Weight: ~ 35 kDa

Pathway: ESC Pluripotency and Differentiation Signaling Pathway.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P48431

Product: Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Research Use Only