Product Description

Sp2 (D512) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS2029-50, BS2029-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q02086

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: IHC

All Applications: IHC: 1:50~1:200

Background: The Sp transcription factor family includes Sp1, Sp2, Sp3 (SPR-2) and Sp4(SPR-1). Sp transcription factors share similar structures but do not share similar functions. All four proteins contain a highly conserved DNA-binding domain composed of three zinc fingers at the C-terminus. Sp family members bind the consensus sequence GGGGCGGGGC and other closely related sequences which are known as GC boxes. Sp1, Sp3 and Sp4 share a high affinity for GC boxes while Sp2 does not. Sp2 only weakly binds to GT boxes. Sp1, Sp2 and Sp3 are ubiquitously expressed, while Sp4 is abundantly expressed in brain with limited expression in other tissues. Sp1 and Sp3, but not Sp2 or Sp4, interact with E2, a regulatory element for the b4 subunit of neuronal nicotinic acetylcholine receptors. Sp3 is the only Sp member to inhibit Sp1 and Sp4 mediated transcription. The gene3 encoding human Sp2 maps to chromosome 17p32.3.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemistry (IHC) analyzes of Sp2 (D512) pAb in paraffin-embedded human colon carcinoma tissue at 1:50. showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody,Secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Molecular Weight: ~ 64 kDa

Specificity: Sp2 (D512) polyclonal antibody detects endogenous levels of Sp2 protein.

Alternative Name: Transcription factor Sp2; KIAA0048; SP2

Immunogen: Synthetic peptide, corresponding to amino acids 500-550 of Human Sp2.

Conjugate: Unconjugated

Modification: Unmodified