Product Description

SPARC Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS6738-50, BS6738-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: SPARC (for secreted protein acidic and rich in cysteine) is a phosphorylated, acidic, glycine-rich glycoprotein that is secreted by endothelial cells and is present in large amounts in the parietal endoderm of mouse embryos and in human placenta. It is identical to osteonectin, a protein important to bone calcification that is highly conserved between species. SPARC, which can be selectively expressed by the endothelium in response to certain types of injury, induces rounding in adherent endothelial cells in vitro. It regμlates endothelial barrier function through F-Actin-dependent changes in cell shape, coincident with the appearance of intercellμlar gaps, which provide a paracellμlar pathway for extravasation of macromolecμles.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC-P, IF/ICC

Alternate Names: SPARC, Basement-membrane protein 40, BM-40, Osteonectin, Short name=ON, Secreted protein acidic and rich in cysteine, SPARC, ON

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodified

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 18-303 of human SPARC (NP_003109.1).

Dilution: WB 1:500 - 1:2000
IHC-P 1:50 - 1:100
IF /ICC 1:50 - 1:100

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: SPARC polyclonal antibody detects endogenous levels of SPARC protein.

Molecular Weight: ~ 35 kDa

Pathway: Contribution of ECM and Cytoskeletal Factors to EMT,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P09486

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only