SQSTM1 Mouse Monoclonal Antibody-MB9009

SQSTM1 Mouse Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB9009-50, MB9009-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Swiss-Prot: Q13501

Host: Mouse

Reactivity: Human, Monkey, Mouse, Rat

Applications: FC, IHC, WB,

All Applications: WB 1:500~2000
IHC 1:150
FLOW 1:100

Background: Sequestosome 1 (SQSTM1, p62) is a ubiquitin binding protein involved in cell signaling, oxidative stress and autophagy. It was first identified as a protein that binds to the SH2 domain of p56Lck and independently found to interact with PKCζ. SQSTM1 was subsequently found to interact with ubiquitin, providing a scaffold for several signaling proteins and triggering degradation of proteins through the proteasome or lysosome. Interaction between SQSTM1 and TRAF6 leads to the K63-linked polyubiquitination of TRAF6 and subsequent activation of the NF-κB pathway. It may play a role in titin/TTN downstream signaling in muscle cells and regulate signaling cascades through ubiquitination. P62 may be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels.

Purification and Purity: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of SQSTM1 and does not cross-react with related proteins.

Bioworld Molecular Weight: ~ 62 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of SQSTM1 Mouse mAb at 1:500 dilution, Immunohistochemical analysis of paraffin-embedded Human pancreas tissue using anti-SQSTM1 antibody. Counter stained with hematoxylin., Immunohistochemical analysis of paraffin-embedded Human thyroid tissue using anti-SQSTM1 antibody. Counter stained with hematoxylin., Immunohistochemical analysis of paraffin-embedded Human prostate tissue using anti-SQSTM1 antibody. Counter stained with hematoxylin.

Alternative Name: Sequestosome-1; EBI3-associated protein of 60 kDa; EBIAP; p60; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; Ubiquitin-binding protein p62; SQSTM1; ORCA; OSIL

Immunogen: Full length human recombinant protein of human SQSTM1 (NP_003891) produced in HEK293T cell.

Conjugate: Unconjugated

Modification: Unmodified