Product Description

Sucrose Colorimetric Assay Kit

Sizes: 50Assays, 100Assays

Catalogue Numbers: E-BC-K161-S-50, E-BC-K161-S-100

Lead times: 2-3 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: Sucrose in plant tissue is hydrolyzed to glucose and fructose in boiling water bath under acidic conditions. 5-hydroxymethyl furfural was synthesized from fructose under acid condition and measure the ultraviolet absorption of 5-hydroxymethyl furfural. Glucose must be dissimilated into ketose structure and reduced to obtain 5-hydroxymethylfurfural, but the rate of isomerization of glucose to ketose is very slow. Therefore, the ultraviolet absorption of glucose is much smaller than fructose.

Research Areas: Glycolysis and Lipid Metabolism

Result Type: Quantitative

Detection Instrument: Spectrophotometer

Detection Type: Colorimetric method

Detection Range: 0.32-70 μmol/mL

Tested Sample Types: Plant tissue

Assay Length: 40 min

Sensitivity: 0.32 μmol/mL

Inter cv: 8.5%

Intra cv: 3.4%

Research Use Only