Sucrose Fluorometric Assay Kit - E-BC-F042

Sucrose Fluorometric Assay Kit

Size: 96T

Catalogue Number: E-BC-F042-96

Citations, Manuals and MSDS Available upon request.

Research Areas: Glycolysis and Lipid Metabolism;Plant Physiology Series;Glycolysis and Lipid Metabolism

Result Type: Quantitative

Detection Instrument: Fluorescence microplate reader

Tested Sample Types: Plant tissue

Assay Time: 70 min

Detection Type: Fluorescence method

Sensitivity: 0.15 μmol/L

Detection Range: 0.15-15 μmol/L

Test Principle: Sucrose can be hydrolyzed by sucrase to produce glucose under acidic conditions, which is catalyzed by glucose oxidase to produce hydrogen peroxide. In the presence of HRP (horse radish peroxidase) , hydrogen peroxide reacts with the fluorescent probe to form red fluorescent substance. The sucrose content can be calculated by measuring the fluorescence intensity at the excitation wavelength of 535 nm and the emission wavelength of 590 nm.

Inter CV: 6.5

Intra CV: 2.3

Storage: This product can be stored at -20°C for 12 months with shading light.

Shipping: Ice bag