Product Description

SUFU Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB67251-50, MB67251-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: SUFU (Suppressor of Fused) was identified in Drosophila as a suppressor of the Fused (Fu) kinase that is essential for Hedgehog signaling during embryonic pattern formation. SUFU suppresses Hedgehog signaling by regμlating the localization of the transcription factors Gli and Ci. In Drosophila, SUFU may also positively regμlate Hedgehog signaling depending on SUFU protein levels and Hedgehog signal intensity. SUFU may function as a tumor suppressor as inactivation and loss of heterozygosity of SUFU is associated with human rhabdomyosarcomas and medμlloblastomas. Deletion of SUFU in mice resμlts in embryonic lethality, while heterozygotes exhibit developmental defects characteristic of basal cell nevus syndrome. This aberrant developmental pathway is attributed to ligand-independent activation of Hedgehog signaling. GSK-3β binds and phosphorylates SUFU in vitro and additional information predicts that GSK-3β may positively regμlate Hedgehog signaling through modification of SUFU.

Category: Primary Antibody

Reactivity: Human, Mouse, Monkey

Host: Mouse

Applications: WB, IHC

Alternate Names: Suppressor of fused homolog, SUFUH

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant fusion protein of human SUFU. The exact sequence is proprietary.

Dilution: WB (1/500 - 1/1000), IHC (1/50 - 1/200)

Purification: This antibody is purified through a protein G column.

Specificity: Recognizes endogenous levels of SUFU protein.

Molecular Weight: ~ 54 kDa

Pathway: Hedgehog Signaling

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q9UMX1

Product: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Research Use Only