Product Description

Survivin (phospho Thr34) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES5788-50, ES5788-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors, yet low in adult tissues. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jun 2011],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, ELISA, IHC

Alternate Names: BIRC5, API4, IAP4, Baculoviral IAP repeat-containing protein 5, Apoptosis inhibitor 4, Apoptosis inhibitor survivin

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human Survivin around the phosphorylation site of Thr34. AA range:11-60

Isotype: IgG

Target: Survivin

Specificity: Phospho-Survivin (T34) Polyclonal Antibody detects endogenous levels of Survivin protein only when phosphorylated at T34.

Dilution: WB 1:500-2000, IHC-p 1:50-300, ELISA 2000-20000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Expressed only in fetal kidney and liver, and to lesser extent, lung and brain (PubMed:10626797), Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas (PubMed:14741722, PubMed:16329164), Also expressed in various renal cell carcinoma cell lines (PubMed:10626797), Expressed in cochlea including the organ of Corti, the lateral wall, the interdental cells of the Limbus as well as in Schwann cells and cells of the cochlear nerve and the spiral ganglions (at protein level), Not expressed in cells of the inner and outer sulcus or the Reissner's membrane (at protein level) (PubMed:21364656, PubMed:20627126),

Observed Band (KD): 16kD

Fields: Platinum drug resistance, Apoptosis, Apoptosis - multiple species, Hippo signaling pathway, Hepatitis B, Pathways in cancer, Chemical carcinogenesis - receptor activation, Colorectal cancer

Function: Domain: The BIR repeat is necessary and sufficient for HBXIP binding
Function: May play a role in neoplasia. May counteract a default induction of apoptosis in G2/M phase. Interacts with tubulin. Inhibitor of caspase-3 and caspase-7. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Isoforms 2 and 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform
Similarity: Belongs to the IAP family
Similarity: Contains 1 BIR repeat
Subcellular Location: Localizes on chromosome arms and inner centromeres from prophase through metaphase and t

Subcellular Location: Cytoplasm Nucleus Chromosome Chromosome, centromere Cytoplasm, cytoskeleton, spindle Chromosome, centromere, kinetochore Midbody Localizes at the centromeres from prophase to metaphase, at the spindle midzone during anaphase and a the midbody during telophase and cytokinesis Accumulates in the nucleus upon treatment with leptomycin B (LMB), a XPO1/CRM1 nuclear export inhibitor (By similarity) Localizes on chromosome arms and inner centromeres from prophase through metaphase Localizes to kinetochores in metaphase, distributes to the midzone microtubules in anaphase and at telophase, localizes exclusively to the midbody (PubMed:11084331) Colocalizes with AURKB at mitotic chromosomes (PubMed:14610074) Acetylation at Lys-129 directs its localization to the nucleus by enhanci

Storage and Stability: -20°C/1 year

Gene Name: BIRC5

Protein Name: Baculoviral IAP repeat-containing protein 5

Mouse Gene ID: 11799

Mouse SwissProt Number: O70201

Rat Gene ID: 64041

Rat SwissProt Number: Q9JHY7

Research Use Only