Product Description

Synaptotagmin 1/2 (phospho Thr202/199) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES1461-50, ES1461-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin-1 participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]).[supplied by OMIM, Jul 2010],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: SYT1, SVP65, SYT, Synaptotagmin-1, Synaptotagmin I, SytI, p65, SYT2, Synaptotagmin-2, Synaptotagmin II, SytII

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human Synaptotagmin around the phosphorylation site of Thr202. AA range:176-225

Isotype: IgG

Target: Synaptotagmin 1/2

Specificity: Phospho-Synaptotagmin 1/2 (T202/199) Polyclonal Antibody detects endogenous levels of Synaptotagmin 1/2 protein only when phosphorylated at T202/199.

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Expressed in melanocytes (PubMed:23999003),

Observed Band (KD): 60kD

Fields: Synaptic vesicle cycle

Function: Cofactor: Binds 3 calcium ions per subunit. The ions are bound to the C2 domains
Domain: The first C2 domain mediates Ca(2+)-dependent phospholipid binding
Domain: The second C2 domain mediates interaction with SV2A and STN2
Function: May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner, these are neurexins, syntaxin and AP2
Similarity: Belongs to the synaptotagmin family
Similarity: Contains 2 C2 domains
Subcellular Location: Synaptic vesicles and chromaffin granules
Subunit: H

Subcellular Location: Cytoplasmic vesicle, secretory vesicle membrane, Single-pass membrane protein Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane, Single-pass membrane protein Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane, Single-pass membrane protein Cytoplasm

Storage and Stability: -20°C/1 year

Gene Name: SYT1/SYT2

Protein Name: Synaptotagmin-1/2

Mouse Gene ID: 20979/20980

Rat Gene ID: 25716/24805

Rat SwissProt Number: P21707/P29101

Research Use Only