Product Description

Syntaxin 1 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES4170-50, ES4170-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes a member of the syntaxin superfamily. Syntaxins are nervous system-specific proteins implicated in the docking of synaptic vesicles with the presynaptic plasma membrane. Syntaxins possess a single C-terminal transmembrane domain, a SNARE [Soluble NSF (N-ethylmaleimide-sensitive fusion protein)-Attachment protein REceptor] domain (known as H3), and an N-terminal regulatory domain (Habc). Syntaxins bind synaptotagmin in a calcium-dependent fashion and interact with voltage dependent calcium and potassium channels via the C-terminal H3 domain. This gene product is a key molecule in ion channel regulation and synaptic exocytosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: STX1A, STX1, Syntaxin-1A, Neuron-specific antigen HPC-1

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from the Internal region of human STX1A. AA range:31-80

Isotype: IgG

Target: Syntaxin 1

Specificity: Syntaxin 1 Polyclonal Antibody detects endogenous levels of Syntaxin 1 protein.

Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: [Isoform 1]: Highly expressed in embryonic spinal cord and ganglia and in adult cerebellum and cerebral cortex,, [Isoform 2]: Expressed in heart, liver, fat, skeletal muscle, kidney and brain,

Observed Band (KD): 30kD

Fields: SNARE interactions in vesicular transport, Synaptic vesicle cycle, Insulin secretion, Huntington disease, Pathways of neurodegeneration - multiple diseases, Amphetamine addiction

Function: Disease: Haploinsufficiency of STX1A may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in Williams-Beuren syndrome (WBS), a rare developmental disorder. It is a contiguous gene deletion syndrome involving genes from chromosome band 7q11.23
Function: Potentially involved in docking of synaptic vesicles at presynaptic active zones. May play a critical role in neurotransmitter exocytosis
Similarity: Belongs to the syntaxin family
Similarity: Contains 1 t-SNARE coiled-coil homology domain
Subunit: Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A. This complex binds to CPLX1. Binds SYTL4 and STXBP6. Found in a ternary complex with STX1A and SNAP25. Interacts with OTOF (By similarity). Found in a complex with VAMP8 and SNAP23. Interacts with VAPA and SYBU
Tissue Specificity: Isoform 1 is highly expressed in embryonic spinal chord and ganglia

Subcellular Location: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane, Single-pass type IV membrane protein Cell junction, synapse, synaptosome Cell membrane Colocalizes with KCNB1 at the cell membrane, [Isoform 2]: Secreted

Storage and Stability: -20°C/1 year

Gene Name: STX1A

Protein Name: Syntaxin-1A

Mouse Gene ID: 20907

Mouse SwissProt Number: O35526

Rat Gene ID: 116470

Rat SwissProt Number: P32851

Research Use Only