Product Description

TAP-Tag Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS65488M-50, BS65488M-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Tandem affinity purification (TAP) is a purification technique for studying protein–protein interactions. It involves creating a fusion protein with a designed piece, the TAP tag, on the end. The original TAP method involves the fusion of the TAP tag to the C-terminus of the protein under study. The TAP tag consists of calmodμlin binding peptide (CBP) from the N-terminal, followed by tobacco etch virus protease (TEV protease) cleavage site and Protein A, which binds tightly to IgG. The relative order of the modμles of the tag is important because Protein A needs to be at the extreme end of the fusion protein so that the entire complex can be retrieved using an IgG matrix.

Category: Primary Antibody

Reactivity: TAP-Tag

Host: Mouse

Applications: WB

Alternate Names: TAP Tag, TAPTag, Tandem affinity purification

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant TAP-Tag

Dilution: WB=1:1000-5000

Purification: affinity purified by Protein G

Specificity: TAP-tag monoclonal antibody detects over-expressed or recombinant proteins containing the TAP epitope tag.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Research Use Only