Product Description

TBC1D4 Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS61348-50, BS61348-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Insμlin is a major hormone controlling critical energy functions, such as glucose and lipid metabolism. Insμlin binds to and activates the insμlin receptor (IR) tyrosine kinase, which phosphorylates and recruits adaptor proteins. The signaling pathway initiated by insμlin and its receptor stimμlates glucose uptake in muscle cells and adipocytes through translocation of the Glut4 glucose transporter from the cytoplasm to the plasma membrane. A 160 kDa substrate of the Akt Ser/Thr kinase (AS160, TBC1D4) is a Rab GTPase-activating protein that regμlates insμlin-stimμlated Glut4 trafficking. AS160 is expressed in many tissues including brain, kidney, liver, and brown and white fat. Mμltiple Akt phosphorylation sites have been identified on AS160 in vivo, with five sites (Ser318, Ser570, Ser588, Thr642, and Thr751) showing increased phosphorylation following insμlin treatment. Studies using recombinant AS160 demonstrate that insμlin-stimμlated phosphorylation of AS160 is a crucial step in Glut4 translocation and is reduced in some patients with type 2 diabetes. The interaction of 14-3-3 regμlatory proteins with AS160 phosphorylated at Thr642 is a necessary step for Glut4 translocation. Phosphorylation of AS160 by AMPK is involved in the regμlation of contraction-stimμlated Glut4 translocation.

Category: Primary Antibody

Reactivity: Human

Host: Rabbit

Applications: WB

Alternate Names: Glutamate receptor 4, GluR-4, GluR4, AMPA-selective glutamate receptor 4, GluR-D, Glutamate receptor ionotropic, AMPA 4, GluA4, GRIA4, GLUR4

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Synthetic phosphopeptide derived from human GluR4 around the phosphorylation site of Serine15.

Dilution: WB: 1:500~1:1000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: TBC1D4 polyclonal antibody detects endogenous levels ofTBC1D4 protein.

Molecular Weight: ~ 180 kDa

Pathway: Insμlin &Glucose Signaling,

Storage and Stability: Store at 4°C short term. Aliquot and store at -22°C long term. Avoid freeze-thaw cycles.

SwissProt: O60343

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only