TBC1D4 Polyclonal Antibody - BT-AP08853

TBC1D4 Polyclonal Antibody

Sizes: 20μL, 50μL, 100μL

Catalogue Numbers: BT-AP08853-20, BT-AP08853-50, BT-AP08853-100

Citations, Manuals and MSDS Available upon request.

Background: TBC1D4 (TBC1 domain family member 4) is a member of the Tre-2/BUB2/CDC16 domain family. The protein encoded by TBC1D4 is a Rab-GTPase-activating protein, and contains two phopshotyrosine-binding domains (PTB1 and PTB2), a calmodulin-binding domain (CBD), a Rab-GTPase domain, and multiple AKT phosphomotifs. This protein is thought to play an important role in glucose homeostasis by regulating the insulin-dependent trafficking of the glucose transporter 4 (GLUT4), important for removing glucose from the bloodstream into skeletal muscle and fat tissues. Reduced expression of this gene results in an increase in GLUT4 levels at the plasma membrane, suggesting that this protein is important in intracellular retention of GLUT4 under basal conditions. When exposed to insulin, this protein is phosphorylated, dissociates from GLUT4 vesicles, resulting in increased GLUT4 at the cell surface, and enhanced glucose transport. Phosphorylation of this protein by AKT is required for proper translocation of GLUT4 to the cell surface. Individuals homozygous for a mutation in this gene are at higher risk for type 2 diabetes and have higher levels of circulating glucose and insulin levels after glucose ingestion. Alternative splicing results in multiple transcript variants encoding different isoforms.

Research Areas: Signal Transduction

Target Protein: TBC1D4

Applications: IHC-p, IF, ELISA

Reactivity: Human, Mouse

Clonality: Polyclonal

Host: Rabbit

Isotype: IgG

Full Product Name: TBC1D4 Antibody

Immunogen: The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. AA range:140-189

Storage: -20°C for 1 year

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration: 1 mg/ml

Molecular Weight (Da): 146563

UniProt Accession: Human: O60343; Mouse: Q8BYJ6

Synonyms: TBC1D4; AS160; KIAA0603; TBC1 domain family member 4; Akt substrate of 160 kDa; AS160

GeneID: Human: 9882; Mouse: 210789

Research Use Only