Product Description

TCP-1 ε Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES5278-50, ES5278-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Mutations in this gene cause hereditary sensory and autonomic neuropathy with spastic paraplegia (HSNSP). Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 5 and 13. [provided by RefSeq, Apr 2015],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, ELISA

Alternate Names: CCT5, CCTE, KIAA0098, T-complex protein 1 subunit epsilon, TCP-1-epsilon, CCT-epsilon

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human CCT5. AA range:241-290

Isotype: IgG

Target: TCP-1 ε

Specificity: TCP-1 ε Polyclonal Antibody detects endogenous levels of TCP-1 ε protein.

Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Bone marrow, Brain, Cajal-Retzius cell, Embryonic kidney, Fetal brain c

Observed Band (KD): 67kD

Function: Disease: Defects in CCT5 are the cause of autosomal recessive sensory neuropathy with spastic paraplegia [MIM: 256840]. The disease is characterized by spastic paraplegia and progressive distal sensory neuropathy leading to mutilating ulcerations of the upper and lower limbs
Function: Molecular chaperone, assist the folding of proteins upon ATP hydrolysis. Known to play a role, in vitro, in the folding of actin and tubulin
Similarity: Belongs to the TCP-1 chaperonin family
Subunit: Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter. Interacts with PACRG

Subcellular Location: Cytoplasm Cytoplasm, cytoskeleton, microtubule organizing center, centrosome

Storage and Stability: -20°C/1 year

Gene Name: CCT5

Protein Name: T-complex protein 1 subunit epsilon

Mouse Gene ID: 12465

Mouse SwissProt Number: P80316

Rat Gene ID: 294864

Rat SwissProt Number: Q68FQ0

Research Use Only