Product Description

TERT Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES4244-50, ES4244-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced variants encoding different isoforms of telomerase reverse transcriptase have been identified, the full-length sequence of some variants has not been determined. Alternative sp

Reactivity: Human, Rat, Mouse,

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: TERT, EST2, TCS1, TRT, Telomerase reverse transcriptase, HEST2, Telomerase catalytic subunit, Telomerase-associated protein 2, TP2

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from the C-terminal region of human TERT. AA range:931-980

Isotype: IgG

Target: TERT

Specificity: TERT Polyclonal Antibody detects endogenous levels of TERT protein.

Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Expressed at a high level in thymocyte subpopulations, at an intermediate level in tonsil T-lymphocytes, and at a low to undetectable level in peripheral blood T-lymphocytes,

Observed Band (KD): 130kD

Fields: Human papillomavirus infection, Human T-cell leukemia virus 1 infection, Pathways in cancer, Hepatocellular carcinoma, Gastric cancer

Function: Catalytic Activity: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Disease: Activation of telomerase has been implicated in cell immortalization and cancer cell pathogenesis
Disease: Defects in TERT are a cause of dyskeratosis congenita autosomal dominant (ADDKC) [MIM: 127550], also known as dyskeratosis congenita Scoggins type. ADDKC is a rare, progressive bone marrow failure syndrome characterized by the triad of reticulated skin hyperpigmentation, nail dystrophy, and mucosal leukoplakia. Early mortality is often associated with bone marrow failure, infections, fatal pulmonary complications, or malignancy
Disease: Defects in TERT are associated with susceptibilty to aplastic anemia (AA) [MIM: 609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is

Subcellular Location: Nucleus, nucleolus Nucleus, nucleoplasm Nucleus Chromosome, telomere Cytoplasm Nucleus, PML body Shuttling between nuclear and cytoplasm depends on cell cycle, phosphorylation states, transformation and DNA damage Diffuse localization in the nucleoplasm Enriched in nucleoli of certain cell types Translocated to the cytoplasm via nuclear pores in a CRM1/RAN-dependent manner involving oxidative stress-mediated phosphorylation at Tyr-707 Dephosphorylation at this site by SHP2 retains TERT in the nucleus Translocated to the nucleus by phosphorylation by AKT

Storage and Stability: -20°C/1 year

Gene Name: TERT

Protein Name: Telomerase reverse transcriptase

Mouse SwissProt Number: O70372

Research Use Only