Product Description

TFAM Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS7319-50, BS7319-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q00059

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF/ICC, ChIP,

All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:200 | IF/ICC,1:50 - 1:200 | ChIP,1:50 - 1:200

Background: This gene encodes a key mitochondrial transcription factor containing two high mobility group motifs. The encoded protein also functions in mitochondrial DNA replication and repair. Sequence polymorphisms in this gene are associated with Alzheimer's and Parkinson's diseases. There are pseudogenes for this gene on chromosomes 6, 7, and 11. Alternative splicing results in multiple transcript variants.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 25KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using TFAM antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s., Immunohistochemistry of paraffin-embedded rat brain using TFAM antibody at dilution of 1:100. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded human colon using TFAM antibody at dilution of 1:100. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol., Immunofluorescence analysis of NIH/3T3 cells using TFAM Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: TFAM; MTDPS15; MTTF1; MTTFA; TCF6; TCF6L1; TCF6L2; TCF6L3

Immunogen: A synthetic peptide of human TFAM(NP_003192.1).

Conjugate: Unconjugated

Modification: Unmodified