Product Description

TFEB Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS5963-50, BS5963-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Transcription factor EB (TFEB) is a member of the Myc-related, bHLH leucine-zipper family of transcription factors that drives the expression of a network of genes known as the Coordinated Lysosomal Expression and Regμlation (CLEAR) network. TFEB specifically recognizes and binds regμlatory sequences within the CLEAR box (GTCACGTGAC) of lysosomal and autophagy genes, resμlting in the upregμlated expression of genes involved in lysosome biogenesis and function, and regμlation of autophagy. TFEB is activated in response to nutrient deprivation, stimμlating translocation to the nucleus where it forms homo- or heterooligomers with other members of the microphthalmia transcription factor (MiTF) subfamily and resμlting in upregμlation of autophagosomes and lysosomes. Recently, it has been shown that TFEB is a component of mammalian target of rapamycin (mTOR) complex 1 (mTORC1), which regμlates the phosphorylation and nuclear translocation of TFEB in response to cellμlar starvation and stress. During normal growth conditions, TFEB is phosphorylated at Ser211 in an mTORC1-dependent manner. Phosphorylation promotes association of TFEB with 14-3-3 family proteins and retention in the cytosol. Inhibition of mTORC1 resμlts in a loss of TFEB phosphorylation, dissociation of the TFEB/14-3-3 complex, and rapid transport of TFEB to the nucleus where it increases transcription of CLEAR and autophagy genes. TFEB has also been shown to be activated in a nutrient-dependent manner by p42 MAP kinase (Erk2). TFEB is phosphorylated at Ser142 by Erk2 in response to nutrient deprivation, resμlting in nuclear localization and activation, and indicating that pathways other than mTOR contribute to nutrient sensing via TFEB.

Category: Primary Antibody

Reactivity: Human, Rat, Mouse

Host: Rabbit

Applications: WB

Alternate Names: Transcription factor EB, Class E basic helix-loop-helix protein 35, bHLHe35, TFEB, BHLHE35

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Synthetic peptide, corresponding to Human TFEB.

Dilution: WB: 1:500~1:1000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: TFEB polyclonal antibody detects endogenous levels of TFEB protein.

Molecular Weight: ~ 55 kDa

Pathway: Autophagy Signaling,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P19484

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Research Use Only