TFIIH p44 (T18) Polyclonal Antibody-BS2306

TFIIH p44 (T18) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS2306-50, BS2306-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q13888

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: IHC, IF

All Applications: IHC: 1:50~1:200
IF: 1:50~1:200

Background: Initiation of transcription from protein-coding genes in eukaryotes is a complex process that requires RNA polymerase II, as well as families of basal transcription factors. Binding of the factor TFIID (TBP) to the TATA box is believed to be the first step in the formation of a multiprotein complex containing several additional factors, including TFIIA, TFIIB, TFIIE, TFIIF and TFII. TFIIH (or BTF2) is a multisubunit transcription/DNA repair factor that possesses several enzymatic activities. The core of TFIIH is composed of five subunits, designated p89 (XPB or ERCC3), p62, p52, p44 and p34. Additional subunits of the TFIIH complex are p80 (XPD or ERCC2) and the ternary kinase complex composed of Cdk7, cyclin H and MAT1. Both p89 and p80 have ATP-dependent helicase activity. The p62, p52 and p44 subunits have been shown to be involved in nucleotide excision repair.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemistry (IHC) analyzes of TFIIH p44 (T18) pAb in paraffin-embedded human kidney carcinoma tissue at 1:50. showing nucleus staining. Negative control (the right) Using PBS instead of primary antibody,Secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Molecular Weight: ~ 44 kDa

Specificity: TFIIH p44 (T18) polyclonal antibody detects endogenous levels of TFIIH p44 protein.

Alternative Name: General transcription factor IIH subunit 2; Basic transcription factor 2 44 kDa subunit; BTF2 p44; General transcription factor IIH polypeptide 2; TFIIH basal transcription factor complex p44 subunit; GTF2H2; BTF2P44

Immunogen: Synthetic peptide, corresponding to the N-terminal of Human TFIIH p44.

Conjugate: Unconjugated

Modification: Unmodified