Product Description

TFIIIA Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES5690-50, ES5690-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The product of this gene is a zinc finger protein with nine Cis[2]-His[2] zinc finger domains. It functions as an RNA polymerase III transcription factor to induce transcription of the 5S rRNA genes. The protein binds to a 50 bp internal promoter in the 5S genes called the internal control region (ICR), and nucleates formation of a stable preinitiation complex. This complex recruits the TFIIIC and TFIIIB transcription factors and RNA polymerase III to form the complete transcription complex. The protein is thought to be translated using a non-AUG translation initiation site in mammals based on sequence analysis, protein homology, and the size of the purified protein. [provided by RefSeq, Jul 2008],

Reactivity: Human, Rat, Mouse,

Host: Rabbit

Applications: WB, ELISA

Alternate Names: GTF3A, Transcription factor IIIA, TFIIIA

Clonality: Polyclonal

Immunogen: Synthesized peptide derived from the Internal region of human TFIIIA.

Isotype: IgG

Target: TFIIIA

Specificity: TFIIIA Polyclonal Antibody detects endogenous levels of TFIIIA protein.

Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Ubiquitous,

Observed Band (KD): 41kD

Function: Caution: It is uncertain whether Met-1 is the initiator. Based on the lack of an in-frame AUG codon, mammalian TFIIIA may be translated from this non-AUG initiation site, which has a good Kozak context and which is well conserved among mammals
Function: Interacts with the internal control region (ICR) of approximately 50 bases within the 5S RNA genes, is required for correct transcription of these genes by RNA polymerase III. Also binds the transcribed 5S RNA's. May initiate transcription of the 5S ribosomal RNA gene and maintain the stability of transcription of other genes
Sequence Caution: Translation N-terminally shortened
Similarity: Contains 9 C2H2-type zinc fingers
Tissue Specificity: Ubiquitous

Subcellular Location: Nucleus

Storage and Stability: -20°C/1 year

Gene Name: GTF3A

Protein Name: Transcription factor IIIA

Mouse SwissProt Number: Q8VHT7

Research Use Only