TIMP2 Monoclonal Antibody-MB66898

TIMP2 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB66898-50, MB66898-100

Product: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: P16035

Host: Mouse

Reactivity: Human

Applications: WB, IHC, IF/ICC, FC

All Applications: WB (1/500 - 1/1000), IHC (1/50 - 1/100), IF/ICC (1/10 - 1/50), FC (1/10 - 1/50)

Background: TIMPs are members of the family of tissue inhibitor of matrix metalloproteinases (MMPs) that includes TIMP1, TIMP2, TIMP3, and TIMP4. The main function of TIMPs is their inhibitory effect on MMPs. TIMPs irreversibly inactivate MMPs by direct binding to their catalytic zinc cofactor and resultant inhibition of proteinase function . In addition to MMP inhibition, TIMPs have also been shown to interact with various membrane receptors on the cell surface. Some of these interactions include: TIMP1 with CD63, TIMP2 with 伪3尾1 integrin, and TIMP3 with VEGFR2, all of which result in distinct cellular effects . TIMPs are involved in a wide variety of biological functions, such as tumor angiogenesis and progression , wound healing, and vascular remodeling . Mutations in TIMP3 are associated with Sorsby's fundus dystrophy .

Purification and Purity: This antibody is purified through a protein G column.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of TIMP2 protein.

Bioworld Molecular Weight: ~ 25 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of TIMP2 expression in SW480 (A) whole cell lysates., Immunohistochemical analysis of TIMP2 staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX., Immunofluorescent analysis of TIMP2 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain the cytoplasm (red). DAPI was used to stain the cell nuclei (blue).

Alternative Name: Metalloproteinase inhibitor 2; CSC-21K; Tissue inhibitor of metalloproteinases 2; TIMP-2

Immunogen: Recombinant fusion protein of human TIMP2. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified