TIP47 Monoclonal Antibody-MB66731

TIP47 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB66731-50, MB66731-100

Product: Mouse IgG2a kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: O60664

Host: Mouse

Reactivity: Human

Applications: WB, IF/ICC

All Applications: WB (1/500 - 1/1000), IF/ICC (1/10 - 1/50)

Background: Tail-interacting 47kDa protein (TIP47), known also as human placental tissue protein 17b (PP17b), binds to cytoplasmic domains of the cation-dependent (CD) and cation-independent (CI) mannose 6-phosphate receptors (MPRs) and facilitates their transport from endosomes to the Golgi complex. The inability of TIP47 to bind several proteins also transported from endosomes to the trans Golgi network indicates that TIP47 associates with a very select set of cargo molecules. In CD-MPR, TIP47 recognizes a phenylalanine/tryptophan signal sequence essential for proper sorting within the endosomal pathway.For CI-MPR binding, TIP47 requires cytoplasmic residues 48-74 of CI-MPR for high-affinity binding while residues 75-163 of CI-MPR aid in the presentation of the higher-affinity residues. Additionally, TIP47 competes with AP-2 clathrin adaptor for binding residues 24-29 of CI-MPR. In tissue extracts of cervical carcinoma patients, TIP47 is overexpressed. Dysplastic cells in high grade dysplasias express more TIP47 than dysplastic cells in low grade dysplasias, and both cytoplasmic types of dysplasias express more TIP47 than normal cervical epithelial cells. The gene encoding human TIP47 maps to chromosome 19p13.3.

Purification and Purity: This antibody is purified through a protein G column.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of TIP47 protein.

Bioworld Molecular Weight: ~ 50 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of TIP47 expression in Hela (A), K562 (B), Daudi (C) whole cell lysates., Immunofluorescent analysis of TIP47 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Alternative Name: M6PRBP1; TIP47; Perilipin-3; 47 kDa mannose 6-phosphate receptor-binding protein; 47 kDa MPR-binding protein; Cargo selection protein TIP47; Mannose-6-phosphate receptor-binding protein 1; Placental protein 17; PP17

Immunogen: KLH-conjugated synthetic peptide of human TIP47. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified