Product Description

TIR1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS78132-50, BS78132-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q570C0

Host: Rabbit

Reactivity: Arabidopsis thaliana

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: Encodes an auxin receptor that mediates auxin-regulated transcription. It contains leucine-rich repeats and an F-box and interacts with ASK1, ASK2 and AtCUL1 to form SCF-TIR1, an SCF ubiquitin ligase complex. Related to yeast Grr1p and human SKP2 proteins, involved in ubiquitin-mediated processes. Required for normal response to auxin and repressed in response to flagellin. As part of the SCF complex and in the presence of auxin, TIR1 interacts with Aux/IAA transcriptional repressor proteins and mediates their degradation. Mutations in TIR1 block auxin stimulation of flavonoid synthesis.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of Inflorescences, using TIR1 antibody at 1:1660 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.

Bioworld Molecular Weight: 67KDa

Specificity: Unmodification

Alternative Name: AtTIR1; TRANSPORT INHIBITOR RESPONSE 1

Immunogen: A synthetic peptide of arabidopsis thaliana TIR1(NP_567135.1).

Conjugate: Unconjugated

Modification: Unmodified