Topo IIBeta Polyclonal Antibody - BT-AP09135

Topo IIBeta Polyclonal Antibody

Sizes: 20μL, 50μL, 100μL

Catalogue Numbers: BT-AP09135-20, BT-AP09135-50, BT-AP09135-100

Citations, Manuals and MSDS Available upon request.

Background: TOP2B (topoisomerase (DNA) II beta) encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, beta, is localized to chromosome 3 and the alpha form is localized to chromosome 17. TOP2B encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in TOP2B have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. Alternative splicing of TOP2B results in two transcript variants; however, the second variant has not yet been fully described.

Research Areas: Epigenetics and Nuclear Signaling

Target Protein: TOP2B

Applications: WB, IHC-p, ELISA

Reactivity: Human, Mouse

Clonality: Polyclonal

Host: Rabbit

Isotype: IgG

Full Product Name: Topo IIbeta Antibody

Immunogen: The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. AA range:140-189

Storage: -20°C for 1 year

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration: 1 mg/ml

Molecular Weight (Da): 183267

UniProt Accession: Human: Q02880; Mouse: Q64511

Synonyms: TOP2B; DNA topoisomerase 2-beta; DNA topoisomerase II; beta isozyme

GeneID: Human: 7155; Mouse: 21974

Research Use Only