Product Description

TPX2 Polyclonal Antibody

Sizes: 60μL, 120μL, 200μL

Catalogue Numbers: E-AB-92021-60, E-AB-92021-120, E-AB-92021-200

Citations, Manuals and MSDS Available upon request.

Abbreviation: TPX2

Target Synonym: DIL2; p100; DIL-2; HCTP4; FLS353; HCA519; REPP86; C20orf1; C20orf2; GD:C20orf1; TPX2

Research Areas: Cancer, Cell Biology, Epigenetics and Nuclear Signaling

Conjugation: Unconjugated

Host: Rabbit

Species Reactivity: Human, Rat

Application: WB, IHC, IF

Isotype: IgG

Clonality: Polyclonal

UNIProt ID: Q9ULW0

Background: Spindle assembly factor required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation. TPX2 is inactivated upon binding to importin-alpha. At the onset of mitosis, GOLGA2 interacts with importin-alpha, liberating TPX2 from importin-alpha, allowing TPX2 to activates AURKA kinase and stimulates local microtubule nucleation.

Concentration: 1mg/mL

Immunogen: Recombinant fusion protein of human TPX2

Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.

Purification Method: Affinity purification

Dilution: WB 1:500-1:2000, IHC 1:50-1:200, IF 1:50-1:200

Calculated MW: 85kDa

Observed MW: 105KDa

Storage: -20°C/One year. Avoid freeze / thaw cycles.

Shipping: Ice bag

Research Use Only