Product Description

TriMethyl-Histone H3-K4 Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS7232-50, BS7232-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails, instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat, Other (Wide Range)

Host: Rabbit

Applications: DB, WB, IHC, ChIP, ChIP-seq

Alternate Names: H3.4, H3/g, H3FT, H3t, HIST3H3, Histone H3, HIST1H3A

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Methylated

Immunogen: A synthetic trimethylated peptide around K4 of human histone H3(NP_003520.1).

Dilution: DB, 1:500 - 1:2000 WB, 1:500 - 1:2000 IHC, 1:50 - 1:200 ChIP, 1:20 - 1:100 ChIP-seq, 1:20 - 1:100

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Methylated Antibodies

Molecular Weight: 17KDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q16695

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only