Product Description

Trk A (phospho Tyr680/Y681) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES6421-50, ES6421-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes a member of the neurotrophic tyrosine kinase receptor (NTKR) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. The presence of this kinase leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in this gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, mental retardation and cancer. Alternate transcriptional splice variants of this gene have been found, but only three have been characterized to date. [provided by RefSeq, Jul 2008],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, ELISA

Alternate Names: NTRK1, MTC, TRK, TRKA, High affinity nerve growth factor receptor, Neurotrophic tyrosine kinase receptor type 1, TRK1-transforming tyrosine kinase protein, Tropomyosin-related kinase A, Tyrosine kinase receptor, Tyrosine kinase receptor A,

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human Trk A around the phosphorylation site of Tyr680 and Tyr681. AA range:646-695

Isotype: IgG

Target: Trk A

Specificity: Phospho-Trk A (Y680/Y681) Polyclonal Antibody detects endogenous levels of Trk A protein only when phosphorylated at Y680/Y681.

Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Isoform TrkA-I is found in most non-neuronal tissues, Isoform TrkA-II is primarily expressed in neuronal cells, TrkA-III is specifically expressed by pluripotent neural stem and neural crest progenitors,

Observed Band (KD): 140-180kD

Fields: MAPK signaling pathway, Ras signaling pathway, Calcium signaling pathway, PI3K-Akt signaling pathway, Apoptosis, Neurotrophin signaling pathway, Inflammatory mediator regulation of TRP channels, Pathways in cancer, Transcriptional misregulation in cancer, Thyroid cancer, Central carbon metabolism in cancer

Function: Alternative Products: Both isoforms have similar biological properties,Catalytic Activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Caution: The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data
Disease: Chromosomal aberrations involving NTRK1 are a cause of thyroid papillary carcinoma (PACT) [MIM: 188550]. Intrachromosomal rearrangement that links the protein kinase domain of NTRK1 to the 5'-end of the TPR gene forms the fusion protein TRK-T1. TRK-T1 is a 55 kDa protein reacting with antibodies against the C-terminus of the NTRK1 protein
Disease: Chromosomal aberrations involving NTRK1 are a cause of thyroid papillary carcinoma (PACT) [MIM: 188550]. Translocation t(1, 3)(q21, q11) with TFG generates the TRKT3 (TRK-T3) transcript by fusing TFG to the 3'-end of NTRK1, a rearrangement with TPM3 gen

Subcellular Location: Cell membrane, Single-pass type I membrane protein Early endosome membrane, Single-pass type I membrane protein Late endosome membrane, Single-pass type I membrane protein Recycling endosome membrane, Single-pass type I membrane protein Rapidly internalized after NGF binding (PubMed:1281417) Internalized to endosomes upon binding of NGF or NTF3 and further transported to the cell body via a retrograde axonal transport Localized at cell membrane and early endosomes before nerve growth factor (NGF) stimulation Recruited to late endosomes after NGF stimulation Colocalized with RAPGEF2 at late endosomes

Storage and Stability: -20°C/1 year

Gene Name: NTRK1

Protein Name: High affinity nerve growth factor receptor

Mouse Gene ID: 18211

Mouse SwissProt Number: Q3UFB7

Rat Gene ID: 59109

Rat SwissProt Number: P35739

Research Use Only