Troponin I3 (G136) Polyclonal Antibody-BS1453

Troponin I3 (G136) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS1453-50, BS1453-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P19429

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: IHC, IF

All Applications: IHC: 1:50~1:200
IF: 1:50~1:200

Background: Troponin I is part of a heteromeric complex playing an important role in the regulation of skeletal and cardiac muscle contraction. It consists of three subunits, troponin I (TnI), troponin T (TnT) and troponin C (TnC). Each subunit is responsible for part of troponin complex function. TnI inhibits ATPase activity of acto myosin and TnT and TnI are present in cardiac muscles in different forms than in skeletal muscles. Only one tissue specific isoform of TnI is described for cardiac muscle tissue (cTnI) and this is expressed only in myocardium.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemistry (IHC) analyzes of Troponin I3 (G136) pAb in paraffin-embedded human kidney carcinoma tissue at 1:50,showing cytoplasmic and membrane staining. Negative control (the right) Using PBS instead of primary antibody,Secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Molecular Weight: ~ 28 kDa

Specificity: TNNI3 (G136) polyclonal antibody detects endogenous levels of TNNI3 protein.

Alternative Name: Troponin I, cardiac muscle; Cardiac troponin I; TNNI3; TNNC1

Immunogen: Synthetic peptide, corresponding to amino acids 100-150 of Human Troponin I3.

Conjugate: Unconjugated

Modification: Unmodified