Product Description

Tsg 101 (E303) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS2347-50, BS2347-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q99816

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
IF: 1:50~1:200

Background: It is now well accepted that the transformation of a normal cell to one that is malignant can result from mutations in genes that encode proteins with key regulatory functions. Examples include the retinoblastoma gene product (Rb p110), p53, VHL and APC. Using a novel cloning strategy that allows the isolation of previously uncharacterized genes encoding selectable recessive phenotypes, an additional tumor suppressor gene has been identified. This gene, termed tsg 101 for tumor susceptibility gene 101, encodes a stathmin binding domain protein. When expression of this growth inhibitory gene is blocked in NIH/3T3 cells using antisense mRNA, the cells exhibit a transformed phenotype and are tumorigenic in SL6 mice.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Tsg 101 (E303) polyclonal antibody detects endogenous levels of Tsg 101 protein.

Bioworld Molecular Weight: ~45 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Tsg 101 (E303) polyclonal antibody in extracts from HEK293A cells., Immunohistochemistry (IHC) analyzes of Tsg 101 (E303) pAb in paraffin-embedded human breast carcinoma tissue at 1:5000. nucleus/cell membrane and cytoplasmic staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of Tsg 101 (E303) pAb in paraffin-embedded human kidney carcinoma tissue at 1:5000. cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Alternative Name: Tumor susceptibility gene 101 protein; ESCRT-I complex subunit TSG101; TSG101

Immunogen: Synthetic peptide, corresponding to amino acids 268-320 of Human Tsg 101.

Conjugate: Unconjugated

Modification: Unmodified