Product Description

Tubulin α Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES4161-50, ES4161-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to the mouse and rat Tuba1 genes. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q.

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: TUBA1A, TUBA3, Tubulin alpha-1A chain, Alpha-tubulin 3, Tubulin B-alpha-1, Tubulin alpha-3 chain, TUBA1B, Tubulin alpha-1B chain, Alpha-tubulin ubiquitousTubulin K-alpha-1, Tubulin alpha-ubiquitous chain, TUBA1C, TUBA6, Tubulin alpha-1C chain, Alpha-tubulin 6, Tubulin alpha-6 chain

Clonality: Polyclonal

Immunogen: Synthesized peptide derived from human Tubulin α around the non-acetylation site of K112.

Isotype: IgG

Target: Tubulin α

Specificity: Tubulin α Polyclonal Antibody detects endogenous levels of Tubulin α protein only when acetylation at K112.

Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Expressed at a high level in fetal brain,

Observed Band (KD): 50kD

Fields: Phagosome, Apoptosis, Tight junction, Gap junction, Alzheimer disease, Parkinson disease, Amyotrophic lateral sclerosis, Huntington disease, Prion disease, Pathways of neurodegeneration - multiple diseases, Pathogenic Escherichia coli infection, Salmonella infection

Function: Disease: Defects in TUBA1A are the cause of lissencephaly type 3 (LIS3) [MIM: 611603]. LIS is characterized by a smooth brain surface due to the absence (agyria) or reduction (pachygyria) of surface convolutions. It is often associated with psychomotor retardation and seizures. LIS3 features include agyria or pachygyria or laminar heterotopia, severe mental retardation, motor delay, variable presence of seizures, and abnormalities of corpus callosum, hippocampus, cerebellar vermis and brainstem
Function: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain
PTM: Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), resp

Subcellular Location: Cytoplasm, cytoskeleton

Storage and Stability: -20°C/1 year

Gene Name: TUBA1A/TUBA1B/TUBA1C

Protein Name: Tubulin alpha-1A chain/Tubulin alpha-1B chain/Tubulin alpha-1C chain

Mouse Gene ID: 22142/22143/22146

Rat Gene ID: 64158/500929/100909441

Rat SwissProt Number: P68370/Q6P9V9/Q6AYZ1

Research Use Only