Product Description

TYK2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS6753-50, BS6753-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P29597

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF/ICC

All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:200 | IF/ICC,1:50 - 1:200

Background: This gene encodes a member of the tyrosine kinase and, more specifically, the Janus kinases (JAKs) protein families. This protein associates with the cytoplasmic domain of type I and type II cytokine receptors and promulgate cytokine signals by phosphorylating receptor subunits. It is also component of both the type I and type III interferon signaling pathways. As such, it may play a role in anti-viral immunity. A mutation in this gene has been associated with hyperimmunoglobulin E syndrome (HIES) - a primary immunodeficiency characterized by elevated serum immunoglobulin E.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 134KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using TYK2 antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 180s., Immunofluorescence analysis of MCF7 cells using TYK2 Rabbit pAb at dilution of 1:150. Blue: DAPI for nuclear staining., Immunofluorescence analysis of NIH/3T3 cells using TYK2 Rabbit pAb at dilution of 1:150. Blue: DAPI for nuclear staining., Immunofluorescence analysis of PC-12 cells using TYK2 Rabbit pAb at dilution of 1:150. Blue: DAPI for nuclear staining.

Alternative Name: TYK2; IMD35; JTK1

Immunogen: Recombinant fusion protein of human TYK2(NP_003322.3).

Conjugate: Unconjugated

Modification: Unmodified