Product Description

UBE2T Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS61700-50, BS61700-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Protein ubiquitination requires the concerted action of the E1, E2, and E3 ubiquitin-conjugating enzymes. Ubiquitin is first activated through ATP-dependent formation of a thiol ester with ubiquitin-activating enzyme E1. The activated ubiquitin is then transferred to a thiol group of ubiquitin-carrier enzyme E2. The final step is the transfer of ubiquitin from E2 to an ε-amino group of the target protein lysine residue, which is mediated by ubiquitin-ligase enzyme E3.Ubiquitin conjugating-enzyme 2T (UBE2T) is an E2 family member responsible for the ATP-dependent ubiquitin tagging of target proteins for degradation. Research studies indicate that UBE2T plays an important role in the Fanconi anemia pathway and that UBE2T expression is required for normal DNA repair through this pathway. Interaction between UBE2T and FANCL appears to stimμlate UBE2T auto monoubiquitination, leading to UBE2T inactivation and negative regμlation of the Fanconi anemia pathway. Additional research details upregμlation of UBE2T expression in breast cancer cells and certain lung carcinomas, suggesting a possible involvement in these malignancies.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB

Alternate Names: Ubiquitin-conjugating enzyme E2 T, Cell proliferation-inducing gene 50 protein, E2 ubiquitin-conjugating enzyme T, Ubiquitin carrier protein T, Ubiquitin-protein ligase T, UBE2T, HSPC150, PIG50

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Synthetic peptide, corresponding Human UBE2T.

Dilution: WB: 1:500~1:1000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: UBE2T polyclonal antibody detects endogenous levels of UBE2T protein.

Molecular Weight: ~ 25 kDa

Pathway: Ubiquitin Proteasome,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q9NPD8

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only