UBE3B Polyclonal Antibody - RA35814

UBE3B Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: RA35814-50, RA35814-100

Citations, Manuals and MSDS Available upon request.

Background: ubiquitin protein ligase E3B (UBE3B) Homo sapiens The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: E1 ubiquitin-activating enzymes, E2 ubiquitin-conjugating enzymes, and E3 ubiquitin-protein ligases. This gene encodes a member of the E3 ubiquitin-conjugating enzyme family which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme and transfers the ubiquitin to the targeted substrates. A HECT (homology to E6-AP C-terminus) domain in the C-terminus of the longer isoform of this protein is the catalytic site of ubiquitin transfer and forms a complex with E2 conjugases. Shorter isoforms of this protein which lack the C-terminal HECT domain are therefore unlikely to bind E2 enzymes. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [pro

Condition: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage Instructions: -20°C/1 year

Recommended dilutions: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

Alternative Names: UBE3B; Ubiquitin-protein ligase E3B

Applications: WB; IHC-p; IF (paraffin section) ; ELISA

Species Cross-Reactivity: Human; Rat; Mouse

GeneID (Human): 89910

Protein MW (KDa): 123

SWISS: Q7Z3V4

Source: Rabbit

Research Use Only